Open Access |Therapeutic Brief
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Gam-COVID-Vac (Sputnik V): a heterologous adenoviral vector-based COVID-19 vaccine
* Corresponding author: Shyh Poh Teo
Mailing address: Geriatrics and Palliative Unit, Department of
Internal Medicine, Raja Isteri Pengiran Anak Saleha (RIPAS)
Hospital, Jalan Putera Al-Muhtadee Billah, Bandar Seri Begawan BA1710, Brunei Darussalam.
E-mail: shyhpoh.teo@moh.gov.bn
Received: 20 April 2021 / Accepted: 23 June 2021
DOI: 10.31491/APT.2021.06.059
Abstract
The Sputnik V COVID-19 vaccine (Gam-COVID-Vac) is an adenoviral vector-based vaccine against the SARS-CoV-2 virus developed by Gamaleya National Research Centre for Epidemiology and Microbiology in Moscow, Russia. It consists of two recombinant adenovirus vectors; type 26 (rAd26) and type 5 (rAd5), with a heterologous approach used to reduce the risk of immune responses against the vector component. Findings from published studies showed that Gam-COVID-Vac was relatively safe, with mostly mild local and systemic reactions reported. Both rAd26-S and rAd5-S had to be administered to develop effective neutralising antibodies. No cross-reactive neutralising antibodies against the viral vector component was identified. The phase 3 trial performed in Russia only demonstrated an efficacy of 91.6% in preventing COVID-19 disease. Common adverse events were flu-like symptoms, injection site reactions, headaches and asthenia, of which the majority were mild. Results from ongoing studies using Gam-COVID-Vac in Belarus, United Arab Emirates and India would be useful to demonstrate generalisability to other population groups. Details of adverse events and ongoing safety surveillance data are awaited before submission for regulatory approval.
Keywords
Adenovirus Vaccines, COVID-19, Russia, vaccines
The coronavirus disease 2019 (COVID-19) pandemic has
caused significant loss of life globally. Older people are
at high risk of developing infections and complications
from the severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2). Therefore, older people should be prioritized to receive COVID-19 vaccines [1]. As vaccines are
being developed, there is an emerging body of evidence
for the different vaccine platforms, which requires review
in terms of vaccine safety and efficacy. This review describes the current evidence available for the Sputnik V
COVID-19 vaccine (Gam-COVID-Vac).
Gam-COVID-Vac is a novel, heterologous adenoviral
vector-based vaccine against the SARS-CoV-2 virus. It
was developed by Gamaleya National Research Centre for
Epidemiology and Microbiology in Moscow, Russia. This
vaccine consists of two recombinant adenovirus vectors; type 26 (rAd26) and type 5 (rAd5). Both vectors carry the
gene for the full-length SARS-CoV-2 spike glycoprotein
(rAD26-S and rAd5-S respectively). The vaccine is manufactured as two different formulations; the frozen Gam-COVID-Vac (0.5mL per dose) and the lyophilized Gam-COVID-Vac-Lyo, which has to be reconstituted in 1.0mL
sterile water for injection. The frozen vaccine is stored at
-18o
C and produced for large-scale use, while the lyophilized can be kept at 2 to 8o
C, allowing delivery to remote
areas of Russia [2].
The recombinant viral vector vaccine platform has been
shown to induce antibody and cell-mediated immune
responses, with known long-term safety data [3]. Other
COVID-19 vaccines utilizing recombinant adenoviruses
include Oxford-AstraZeneca (AZD1222), using a chimpanzee adenovirus (ChAdOx) [4], Janssen using Ad26
alone [5] and CanSino Biologics – Beijing Institute of
Biotechnology using Ad5 alone[6].
Heterologous vaccination with two different viral vectors
can be advantageous because the immune system may
respond to the vector component in addition to the target
antigen. This immune response to the vector component
may result in reduced vaccine effectiveness, particularly
if two doses are required. Therefore, the use of different
viral vectors should theoretically overcome this problem
and improve vaccine efficacy. This heterologous vaccination approach was shown to be effective when used for
the Ebola virus disease vaccine developed in Russia [7].
Thus, it was anticipated that Gam-COVID-Vac would be
an effective vaccine to prevent COVID-19 disease.
In phase 1, participants received an intramuscular dose of
rAd26-S or rAd5-S in the deltoid. For phase 2, a dose of
rAd26-S was administered, followed by rAd5-S on day
21. Gam-COVID-Vac was used at Burdenko Hospital,
while Gam-COVID-Vac-Lyo was administered at Sechenov University. At each locality, 38 volunteers were enrolled; 9 received rAd26-S and 9 received rAd5-s (Phase
1); while 20 received rAd26-S followed by rAD5-S on
day 21 (Phase 2). The majority of the participants were
men in the late 20s or early 30s [2].
In terms of safety data, most local and systemic reactions
were mild. No serious adverse events were reported. The
most common reactions were localized pain at the injection site, fever, headache, fatigue and myalgia; with more
adverse events occurring after the second dose. In the
phase 1 studies, the SARS-CoV-2 receptor binding domain
(RBD) specific IgG was detected on day 14 in 88.9% after
rAd26-S, and 84.2% after rAd5-S. This immune response
increased to 100% on day 21. However, both rAd26-S and
rAd5-S had to be administered to develop neutralizing antibodies at day 42 in 100% of participants. Participants receiving rAd26-S alone had a seroconversion rate of 61.1%
at day 42.
For the phase 2 studies, post-vaccination ELISA titers
were significantly higher than convalescent plasma post
COVID-19 infection at day 28 and 42. Cell-mediated
responses were also detected in all vaccine recipients by
day 28. There were no cross-reactive neutralizing antibodies against the viral vector component. This was demonstrated using the serum of participants on day 28 after
immunization, where neutralizing antibodies to rAd26 did
not cross-react with rAd5 [2].
The phase 3 trial was carried out in Russia only, with 21
977 adults aged above 18 enrolled in the randomized,
double-blind, placebo-controlled study at 25 centers
across Moscow. Participants were randomly assigned
(3:1) to receive Gam-COVID-Vac vaccine or placebo.
The vaccine group received rAd26 and rAd5 vector-based
heterologous prime-boost COVID19 vaccine at least 21
days apart while the placebo group received the vaccine
buffer composition. The efficacy rate was 91.6% from 21
days after the first dose (the day of the second dose), with
16 of 14964 vaccine recipients and 62 of 4902 placebo
recipients contracting COVID-19 disease. This efficacy
was consistent across all age groups, with the oldest group
being above 60 years old.
The most common adverse events reported were flu-like
symptoms, injection site reactions, headaches and asthenia. The majority (94%) of reported adverse events were
mild or Grade 1 symptoms[8].
There were certain limitations with this trial. As the study
was performed in Moscow with the majority (98.5%) of
participants being white, it remains to be seen if these
results could be extrapolated to other population groups.
Results from the ongoing clinical trials using Gam-COVID-Vac in Belarus, United Arab Emirates and India would
be useful to assess generalisability. The phase 3 trial was
only performed with the frozen preparation stored at
-18o
C, with the assumption that the lyophilized formulation provides the same efficacy. The latter has been approved for use by the Ministry of Health of the Russian
Federation for practical ease of global distribution.
There may an under-diagnosis of COVID-19 cases, as
asymptomatic PCR-confirmed COVID-19 cases were not
included in the efficacy analyses. However, similar to other trials, the main outcome was symptomatic COVID-19
disease. Thus no PCR-testing was done beyond 21 days
of the first dose unless participants developed symptoms
suggestive of COVID-19. It would also be useful to have
efficacy data on day 21 and 42 of the trial. This would allow time for an immunologic response to develop after
the first and second dose. In terms of safety data, only a
preliminary report of adverse events following immunization was provided. There were 68 participants who experienced 70 episodes of serious adverse events among more
than 21000 participants, of whom more than 16000 received the vaccine. The authors stated that further details
would be made available in a future publication regarding
these adverse events. This additional information would
be required before submission for regulatory approval.
The main findings from the Phase 3 trial for Gam-COVID-Vac relevant to older people were as follows: There
was a similar efficacy in younger and older people. This
was reassuring, given the theoretical risk of a reduced
vaccine response in older people due to immune senescence [1]. In terms of adverse events, while the data available was not age-stratified, there was only a small number
of serious adverse events identified at the time of interim
analysis.
For COVID-19 vaccines, the Phase 3 studies should demonstrate the effectiveness and that benefits outweigh the
risks to obtain Emergency Use Authorisation (EUA) from
regulatory authorities. Monitoring of efficacy and safety
data usually requires a 2-month median follow-up period
after completion of the full vaccination regimen. For example, as natural immunity to coronavirus infections is
usually short-lived, the significant waning of protective
responses may become noticeable in the second month [9].
After Phase 3 trials, researchers should also strive to optimize vaccine regimens, including dosing intervals for
booster doses. Fractional dosing or lower doses of vaccine with maintained efficacy may allow more people to
be vaccinated. Specific patient groups, such as pregnant
women and children should also be evaluated. Longer
term follow-up is required to assess the durability of protective immune responses and surveillance for adverse
events after immunization. Consideration should also be
given to effective coordination of post-efficacy trial tasks,
such as mass production while maintaining the quality
of vaccines; and coordinating supplies and distribution.
Scaled-up production may cause problems with the vector,
purification process or formulation, which needs to meet international quality standards before use [10].
Finally, there should be ongoing evaluation of vaccine
efficacy against emerging new SARS-CoV-2 variants.
The variants 501Y.V1 (B.1.1.7) in the United Kingdom
and 501Y.V2 (B.1.351) in South Africa have the mutation N501Y in the receptor-binding domain of the spike
protein, contributing to increased transmission. However,
for the 501Y.V2 variant, two additional mutations (E484K
and K417M) in the spike protein provide a potential immune escape from antibodies. Studies have suggested that
there is reduced efficacy of current vaccines, particularly
against the South Africa variant [11]. Thus, the production
of COVID-19 vaccines should be reactive and adapt to
these newly emerging lineages to remain effective against
the main circulating variants.
In conclusion, the Phase 3 studies showed early promising evidence that Gam-COVID-Vac was efficacious in
preventing SARS-CoV-2 infections, including in older
people. Certain limitations in the studies should be taken
into account, with a need for more specific data on older
people and emerging SARS-CoV-2 variants. Post-marketing surveillance for this vaccine is also required for ongoing monitoring of adverse events following vaccination.
Declaration
Conflict of interest
The author declared no conflict of interest.
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