Ultrasound reverses adriamycin-resistance in non-small cell lung cancer via positive regulation of BRAF-activated non-coding RNA (BANCR) expression | Liang | Clinical Surgery Research Communications

Ultrasound reverses adriamycin-resistance in non-small cell lung cancer via positive regulation of BRAF-activated non-coding RNA (BANCR) expression

Yong-gang Liang, Zhen-tian Liu, Shan-xian Guo

Abstract


Objectives: Multi-drug resistance (MDR) remains one of the major obstacles to successful chemotherapy in patients with cancer. BRAF-activated non-coding RNA (BANCR) has been reported to be regulated in non-small cell lung cancer (NSCLC). In the present study, the researchers investigated the effects of ultrasound on BANCR expression, and its possible mechanisms were investigated in vitro and in vivo.

Methods: The Adriamycin-resistance of A549/ADM cells and the transplantation tumor model of A549/ADM in nude mice were established. Real-time PCR was used to quantify BANCR expression in cells and in mice examined with or without ultrasound. P-gp and MRP protein expression were measured by western blot. The cell viability was detected by MTT assay.

Results: ANCR expression was significantly elevated by ultrasound in A549/ADM cells and tumor tissue from xenograft mice. In in vitro experiments, P-gp and MRP levels were markedly reduced by ultrasound. When the cancer cells up-regulated BANCR by transfection of pCDNA-BANCR, P-gp and MRP levels were inhibited. In addition, the down-regulation of BANCR by si-BANCR transfection elevated their expression.


Conclusion: Ultrasound reversed adriamycin-resistance in A549/ADM cells by regulating BANCR expression, and P-gp and MRP regulation were involved in this process.




Subscribe to receive issue release notifications
and newsletters from journals